In order to achieve a complete killing of Vibrio (from a high level e.g. > 1000000) you need at least 5 ppm Halamid (and probably even more when in dirty water). In the lab, without fish at 25 oC, 17 degrees DH (water hardness) and 25 ppt salinity, 2.5 ppm (> 24 hr contact time) or 5 ppm (less than 24 hr) Halamid were required or a complete kill (log 6 reduction) of Vibrio parahaemolyticus.