To achieve a complete killing of Vibrio (from a high level e.g. > 1000000) you need at least 10 ppm Halamid (possibly more at high organic load). In the lab, without fish at 25 oC, 17 degrees DH (water hardness) and 25 ppt salinity, 10 ppm (> 24 hr contact time) or 20 ppm (less than 24 hr) Halamid were required for a complete kill (log 6 reduction) of Vibrio parahaemolyticus.